Development and validation of a new method for determination of topiramate in bulk and pharmaceutical formulation using high performance liquid chromatography UV detection after pre column derivatization

Simple item page
AuthorMohammadbagher Majnoonien
AuthorRonak Jalilien
AuthorBahareh Mohammadien
AuthorShahram Miraghaeeen
AuthorMohammad Taher Bahramien
AuthorSajad Fakhrien
AuthorGholamreza Bahramien
OrcidSajad Fakhri [0000-0001-8265-8284]en
Issued Date2014-12-31en
AbstractIn published high performance liquid chromatographic (HPLC) methods for analysis of topiramate (TPM) in pharmaceutical dosage forms and raw materials Refractive Index Detector (RID) has been used which is not available in many laboratories and has low sensitivity. We described a new, sensitive and simple HPLC method for determination of topiramate in pharmaceutical forms and Invirto dissolution studies which avoids the use of RID detector. The method is based on derivatization of topiramate and an internal standard by reaction with 4‐chloro‐7‐nitrobenzofurazan (NBD‐CL), and reverse‐phase chromatography using phenyl column and spectrophotometer detection at 264 nm. A mixture of phosphate buffer (0.05 M) containing triethylamine (0.1٪ V/V; pH=2.3) and methanol (28:72, V/V) at a flow rate of 2.2 ml/min was used as mobile phase. The analysis performance was studied and the method was shown to be selective and linear for determination of topiramate in pharmaceutical forms and dissolution studies. en
DOIhttps://doi.org/en
KeywordTopiramateen
KeywordPharmaceutical Formulationen
KeywordDerivatizationen
KeywordHPLCen
KeywordDissolution Studyen
PublisherBrieflandsen
TitleDevelopment and validation of a new method for determination of topiramate in bulk and pharmaceutical formulation using high performance liquid chromatography UV detection after pre column derivatizationen
TypeResearch Articleen

Files

Collections

Journal of Reports in Pharmaceutical Sciences