<i>N</i>-(5-Mercapto-1,3,4-Thiadiazol-2-yl)-2-Phenylacetamide Derivatives: Synthesis and <i>In-vitro</i> Cytotoxicity Evaluation as Potential Anticancer Agents

AuthorAhmad Mohammadi-Faranien
AuthorNeda Heidarianen
AuthorAlireza Aliabadien
Issued Date2014-04-30en
AbstractA new series of N-(5-Mercapto-1,3,4-thiadiazol-2-yl)-2-phenylacetamide derivatives (3a-3j) were synthesized via an amidation reaction using EDC and HOBt in acetonitrile solvent at room temperature condition. Chemical structures were characterized by 1H NMR, IR and MS spectroscopic methods and related melting points were also determined. The anticancer activity was evaluated using MTT procedure in-vitro. All compounds were tested against SKNMC (Neuroblastoma), HT-29 (Colon cancer) and PC3 (Prostate cancer) cell lines. According to the toxicological data, none of the synthesized derivatives exerted superior activity than doxorubicin as reference drug. Derivatives with Ortho chlorine (compound 3d), meta methoxy (compound 3h) and meta fluorine (compound 3b) substituents on the phenyl ring exhibited the best cytotoxic activity against SKNMC (IC50 = 4.5 ± 0.035 µM), HT-29 (IC50 = 3.1 ± 0.030 µM) and PC3 (IC50 = 12.6 ± 0.302 µM) cell lines respectively.en
DOIhttps://doi.org/10.22037/ijpr.2014.1503en
KeywordSynthesisen
Keyword1en
Keyword3en
Keyword4-Thiadiazoleen
KeywordAnticanceren
KeywordAmidationen
PublisherBrieflandsen
Title<i>N</i>-(5-Mercapto-1,3,4-Thiadiazol-2-yl)-2-Phenylacetamide Derivatives: Synthesis and <i>In-vitro</i> Cytotoxicity Evaluation as Potential Anticancer Agentsen
TypeOriginal Articleen

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