Association Between Cytokine Gene Polymorphisms and Human Susceptibility to Brucellosis
| Author | Faezeh Kabiri | en |
| Author | Ramazan Rajabnia | en |
| Author | Abazar Pournajaf | en |
| Author | Mehrdad Gholami | en |
| Author | Meysam Hasannejad Bibalan | en |
| Author | Aziz Kassani | en |
| Author | Sajad Yaghoubi | en |
| Author | Abdollah Ardebili | en |
| Orcid | Ramazan Rajabnia [0000-0002-3407-0950] | en |
| Orcid | Abazar Pournajaf [0000-0002-3407-0950] | en |
| Orcid | Aziz Kassani [0000-0001-5188-5249] | en |
| Orcid | Abdollah Ardebili [0000-0003-2386-8366] | en |
| Issued Date | 2017-07-31 | en |
| Abstract | Background: Allelic single nucleotide polymorphisms (SNPs) in cytokine-encoding genes can affect the degree of cytokine production, and may be related to the tendency to infectious illnesses as well as various clinical consequences. Objectives: The aim of this work was to evaluate the possible role of SNPs in the regions of the IL-10 (-592), IL-15 (-367), IL-18 (-656), IL-12 (+1188), IFN-γ (+874), TNF-α (-308), and TNF-β (+252) genes in susceptibility or resistance to brucellosis and its crucial complications. Methods: In a period of one year, 125 patients with acute brucellosis referring to 3 large public teaching hospitals were enrolled in this study. We studied the SNPs of IL-10, IL-15, IL-18, IL-12, IFN-γ, and TNF-α/β genes using the allele specific polymerase chain reaction (AS-PCR) with sequence-specific primers. Results: Frequency of GG genotype in the TNF-α and TNF-β-encoding genes increased significantly by 52% and 31.2% in patient and control groups, respectively. For IFN-γ, TA genotype was found highly enhanced in patients (60%), while the frequency of AA and TT genotypes were higher in controls (23.2% and 26.4%, respectively). The AA and CC SNPs in IL-12 were dominant in both patient (78.4%) and control (14.4%) groups. In the patient group, the GG and TT genotypes had a higher frequency for genes encoding IL-15 (33.6%) and IL-18 (89.6%). Conclusions: Based on the present study, some SNPs within the several cytokine genes, including TNF-α/β (-308/+252), IFN-γ (+874), IL-15 (-367), IL-18 (-656), and IL-12 (+1188) are related to the susceptibility or resistance to brucellosis. In order to approve the biological consequence of our results, additional investigations should be carried out in larger population groups. | en |
| DOI | https://doi.org/10.5812/pedinfect.57274 | en |
| Keyword | Brucellosis | en |
| Keyword | Cytokines | en |
| Keyword | Single Nucleotide Polymorphisms | en |
| Publisher | Brieflands | en |
| Title | Association Between Cytokine Gene Polymorphisms and Human Susceptibility to Brucellosis | en |
| Type | Research Article | en |
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