HMW1<sub>555-914</sub>, HMW2<sub>553-916</sub>, and Hia<sub>585-705 </sub>as Subunit Vaccine Candidates of Nontypeable <i>Haemophilus influenzae</i> Induce Specific Antibody Responses with Bactericidal Activity in Balb/c
| Author | Kazem Abbaszadeh-Goudarzi | en |
| Author | Ghasem Abbaszadeh-Goudarzi | en |
| Author | Mohammad Reza Khorramizadeh | en |
| Author | Saeid Bouzari | en |
| Author | Sassan Rezaie | en |
| Author | Mehdi Davari | en |
| Author | Seyed Davar Siadat | en |
| Author | Ladan Teimoori-Toolabi | en |
| Issued Date | 2018-01-31 | en |
| Abstract | Background: Nontypeable Haemophilus influenzae (NTHi) is responsible for diseases such as otitis media, sinusitis, bronchitis, and pneumonia. Unlike H. influenzae Serotype b (Hib), there is no vaccine against diseases induced by NTHi. High molecular weight1 (HMW1), high molecular weight 2 (HMW2), and H. influenzae type a (Hia) proteins are critical protein adhesions of NTHi with the potentiality to provide the protection. Objectives: The current study aimed at investigating the potential of recombinant HMW1555-914, HMW2553-916, and Hia585-705 proteins as subunit vaccines to induce immune responses after active immunization in Bagg albino (inbred research mouse strain) or Balb/c mice. Methods: HMW1555-914, HMW2553-916, and Hia585-705 amplified by polymerase chain reaction (PCR) were cloned into pET28a. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and the Western blotting analysis were carried out to investigate the characteristics of proteins. Proteins were purified by Ni-nitrilotriacetic acid (Ni-NTA) as a gel matrix. Mice (Balb/c) were immunized subcutaneously with HMW1555-914, HMW2553-916, and Hia585-705 proteins, alone or in binary and ternary combinations. Eventually, specific antibody responses were evaluated by the enzyme-linked immunosorbent assay (ELISA) and serum bactericidal assay (SBA). Results: Antibody responses significantly increased in Balb/c immunized by ternary combination compared with the control group. IgG1/IgG2a ratio indicated that proteins directed immune responses toward T-helper 2. Antisera produced against purified proteins in ternary combination showed the highest bactericidal activity against NTHi strains with the titer of 1:32. Conclusions: The obtained results suggested that HMW1555-914, HMW2553-916, and Hia585-705 adhesins were the potential subunit vaccine candidates of NTHi and after further investigation could be considered for protection against NTHi infections. | en |
| DOI | https://doi.org/10.5812/jjm.58329 | en |
| Keyword | Vaccines | en |
| Keyword | HMW1 Protein | en |
| Keyword | Bacteria | en |
| Keyword | HMW2 Protein | en |
| Keyword | Hia Adhesion | en |
| Keyword | Recombinant Proteins | en |
| Keyword | <i>Haemophilus influenzae</i> | en |
| Publisher | Brieflands | en |
| Title | HMW1<sub>555-914</sub>, HMW2<sub>553-916</sub>, and Hia<sub>585-705 </sub>as Subunit Vaccine Candidates of Nontypeable <i>Haemophilus influenzae</i> Induce Specific Antibody Responses with Bactericidal Activity in Balb/c | en |
| Type | Research Article | en |