Oxidative Stress Induced by Permanent Cerebral Hypoperfusion Improved by Red Grape Seed Extract in Rats

Abstract

Introduction: This study aimed to evaluate the effect of chronic oral administration of red grape seed extract (GSE) on brain oxidative stress induced after permanent bilateral common carotid arteries occlusion (BCCAO) in male adult rats.Methods: Thirty-two adult male Wistar rats (220±20g). The rats were divided randomly into four groups of 8 in each: 1) Sham+Veh; 2) Sham+GSE; 3) Isch+Veh; 4) Isch+GSE. In order to make animal model of permanent cerebral hypoperfusion/ischemia, both carotid arteries were ligated upper and lower and cut bilaterally. The animals in the treatment groups received a dose of 100 mg/kg GSE daily, by oral gavage (PO) for 4 weeks. Malondialdehyde (MDA) levels, Glutathione peroxidase (GPx) activity, and total thiol (–SH) groups were measured in homogenate of cerebral hippocampus and cortex.Results: MDA elevated significantly in rats’ hippocampus and cortex after chronic cerebral hypoperfusion/ischemia when compared with sham rats (P