A Pusher and a Clip Applied to Hind Paws Under Isoflurane Sedation to Evaluate Neuropathic Pain in a CCI Model

AuthorReiko Nobuharaen
AuthorAkihiro Itoen
AuthorMasafumi Nakagawaen
AuthorTatsunori Ikemotoen
AuthorKimimasa Naritaen
AuthorMakoto Nishiharaen
AuthorYukio Kinoshitaen
AuthorYoung-Chang Araien
OrcidYoung-Chang Arai [0000-0002-2689-866X]en
Issued Date2021-12-31en
AbstractRodent behavior assessments have been developed to evaluate pain. However, their fidgety activity and reactivity to human contact make it hard to activate animals in a consistent manner and get uniform and trustworthy responses. The present study was performed on prairie voles (aged 8 weeks). Sham (7 male prairie voles) and chronic constriction injury (CCI) (8 male prairie voles) rodents were investigated before surgery and four and seven days later. Each animal was assessed for nociceptive behavior. Pressure and mechanical threshold tests were conducted by the application of three different pushers to the center of hind paws and arterial clips to the toes while sedated with isoflurane. The CCI affected right lower extremity prominently increased nociceptive behavior scores four and seven days after the experiment, and the CCI affected right hind paw prominently decreased pressure and mechanical threshold tests four and seven days after the experiment . The pressure and mechanical thresholds were relevant to the scorings of nociceptive behavior in CCI model animals.en
DOIhttps://doi.org/10.5812/aapm.118299en
KeywordEvaluation of Chronic Painen
KeywordCCI Animalsen
KeywordClips and Pushersen
KeywordIsoflurane Sedationen
PublisherBrieflandsen
TitleA Pusher and a Clip Applied to Hind Paws Under Isoflurane Sedation to Evaluate Neuropathic Pain in a CCI Modelen
TypeBrief Reporten

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