Comparison of Various Methods for Isolation of <i>Nocardia</i> from Soil
| Author | Masoumeh Rasouli-Nasab | en |
| Author | Mehdi Fatahi-Bafghi | en |
| Author | Shadi Habibnia | en |
| Author | Parvin Heidarieh | en |
| Author | Seyyed Saeed Eshraghi | en |
| Orcid | Shadi Habibnia [0000-0002-6055-7295] | en |
| Issued Date | 2017-02-28 | en |
| Abstract | Background: The genus Nocardia is Gram-positive, aerobic filamentous bacilli and saprophytic micro-organisms that can be isolated from freshwater, salt water, dust and decaying vegetation especially the soil. This study aimed to investigate the several media for to determine a suitable culture media with the ability to better for the isolation of Nocardia from soil. Methods: In this study, 400 soil samples were collected from different areas from Iran. The soil samples were then cultured on the four culture media such as Humic acid vitamin B agar, Paraffin agar, Sabouraud dextrose agar supplemented whit cycloheximide and carbon-free broth containing paraffin rods and incubated at 35°C. All of culture media investigated every 3 days for a month. Colonies suspicious to Nocardia were stained with Gram-stain, acid-fast and partially acid-fast and evaluated for resistance to lysozyme. Results: From 400 soil samples, the number of 62, 10, 28 and 19 strains of Nocardia were isolated by paraffin rods, Humic Acid Vitamin B agar, Paraffin agar and Sabouraud dextrose agar whit cycloheximide, respectively. Most Nocardia strains were isolated using paraffin bait technique. Conclusions: Isolation of Nocardia spp. is enhanced by using the paraffin baiting technique that relies on the selective ability of this micro-organism to metabolize paraffin. | en |
| DOI | https://doi.org/10.5812/zjrms.6107 | en |
| Keyword | Humic Acid Vitamin B Agar | en |
| Keyword | Paraffin Bait Technique | en |
| Keyword | Paraffin Agar | en |
| Keyword | Sabouraud Dextrose Agar | en |
| Keyword | Soil | en |
| Keyword | <i>Nocardia</i> | en |
| Publisher | Brieflands | en |
| Title | Comparison of Various Methods for Isolation of <i>Nocardia</i> from Soil | en |
| Type | Research Article | en |