Analysis of electrophoretic patterns of extracted somatic proteins from Fusarium Solani

Abstract

Abstract Background: Electrophoretic patterns of extracted somatic proteins from fungi can be useful in identification of different species and strains, and also help to find the sources of infection. Objective: To determine the electrophoretic patterns of extracted somatic proteins from fusarium solani. Methods: Fusarium solani stains UAMH 7419 and UAMH 3317, isolated from a patient and air respectively, were used to prepare the somatic extract of young mycelia. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method was used to determine the number and characteristics of protein bands. Findings: The somatic proteins extracted from F.solani UAMH 3317 and UAMH 7419 resulted in 16 and 21 protein bands respectively, with molecular weights of 14 to 100 KD. Conclusion: The patients isolated strain of F.solani showed more bands in SDS-PAGE than the air isolated strain.