Synergistic Effect of Sodium Butyrate and Thalidomide in the Induction of Fetal Hemoglobin Expression in Erythroid Progenitors Derived from Cord Blood CD133 + Cells
| Author | Ali Dehghanifard | en |
| Author | Saied Kaviani | en |
| Author | Mehrdad Noruzinia | en |
| Author | Masoud Soleimani | en |
| Author | Saeid Abroun | en |
| Author | Abbas Hajifathali | en |
| Author | Ali Akbar Pourfathollah | en |
| Author | Yousef Mortazavi | en |
| Author | Mohammad Ahmadvand | en |
| Author | Maryam Mahmoodinia-Meymand | en |
| Author | Zeinab Kaviani | en |
| Author | Majid Farshdousti-Hagh | en |
| Author | Najmaldin Saki | en |
| Issued Date | 2012-09-30 | en |
| Abstract | Background: The use of drugs with the ability to induce production of fetal hemoglobin as a novel therapeutic approach in treating β-Hemoglobinopathies is considered. γ-globin gene expression inducer drugs including sodium butyrate and thalidomide can reduce additional α-globin chains accumulation in erythroid precursors.Materials and Methods: In this experimental study, MACS kit was used to isolate CD133+ cells of umbilical cord blood. Further, the effect of two drugs of thalidomide and sodium butyrate were separately and combined studied on the induction of quantitative expression of β-globin and γ-globin genes in erythroid precursor cells derived from CD133+ stem cells in-vitro. For this purpose, the technique SYBR green Real-time PCR was used.Results: Flow cytometry results showed that approximately 95% of purified cells were CD133+. Real-time PCR results also showed the increased levels of γ-globin mRNA in the cell groups treated with thalidomide, sodium butyrate and combination of drugs as 2.6 and 1.2 and 3.5 times respectively, and for β-globin gene, it is respectively 1.4 and 1.3 and 1.6 times compared with the control group (p | en |
| DOI | https://doi.org/ | en |
| Keyword | CD133+ cells | en |
| Keyword | γ-globin gene | en |
| Keyword | Sodium butyrate | en |
| Keyword | Thalidomide | en |
| Publisher | Brieflands | en |
| Title | Synergistic Effect of Sodium Butyrate and Thalidomide in the Induction of Fetal Hemoglobin Expression in Erythroid Progenitors Derived from Cord Blood CD133 + Cells | en |
| Type | Research Article | en |
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