Adipose-Derived Mesenchymal Stem Cells Secretome Induces Apoptosis in Colon Carcinoma HT-29 Cells
| Author | Mohammad Javad Khodayar | en |
| Author | Fatemeh Rezaei Tazangi | en |
| Author | Azin Samimi | en |
| Author | Hadis Alidadi | en |
| Issued Date | 2022-12-31 | en |
| Abstract | Background: Recent studies have demonstrated that adipose mesenchymal stem cells (AMSCs)-derived secretome (AMSC-Se) has anticancer impacts. Objectives: This study investigated the cytotoxic impacts of AMSC-Se on a colon carcinoma cell (HT-29) line. Methods: The colon cancer cells were exposed to 50 or 100 µg/mL ASMC-Se for 24 hours. MTT test had used to examine the impacts of ASMC-Se on the survival rates of the cells. Caspase activity, mRNA, and protein expression of Bax and Bcl-2 had evaluated to determine apoptosis. Results: ASMC-Se could diminish the survival of the cells concentration-dependently. The mRNA and protein expression of Bax was concentration-dependently elevated, while Bcl-2 expression decreased in the ASMC-Se group compared to the control concentration-dependently (P < 0.05). The caspase-3 and caspase-9 activities were concentration-dependently enhanced (P < 0.05), while the caspase-8 activity did not change with the AMSC-Se. Conclusions: These findings indicate that AMSC-Se effectively prevents cell growth and induces apoptosis by stimulating the intrinsic apoptotic pathway in these cells. | en |
| DOI | https://doi.org/10.5812/jjcmb-133934 | en |
| Keyword | HT-29 Cell Line | en |
| Keyword | Apoptosis | en |
| Keyword | Adipose-Derived Mesenchymal Stem Cells | en |
| Keyword | Secretome | en |
| Publisher | Brieflands | en |
| Title | Adipose-Derived Mesenchymal Stem Cells Secretome Induces Apoptosis in Colon Carcinoma HT-29 Cells | en |
| Type | Research Article | en |