The qualitative study of cell surface and extra cellular matrix markers in benign and malignant lesions of prostate

Abstract

In benign and malignant lesions of prostate, morphologic changes of cell and stroma has occurred simultaneous with changes of cell surface components and extra cellular matrix. It seems that these factors are responsible for cell transformation, metastasis and escape of cancer cells from immune system. New studies showed cell and extra cellular matrix changes in benign and neoplastic lesions in a few organs. The aim of the present study was to identify Gal/Gal Nac in cell and extra cellular matrix of benign and malignant lesions of prostate. 35 paraffin blocks (20 adenocarcinoma and 15 prostatitis and hyperplasia) were chosen from pathology file of Khatam-Alanbia hospital in Zahedan. Sections were stained for 2 hours by lectin histochemistry using PNA/Alcian blue pH=2.5 (lectins diluted 10 microgram/ml in PBS 0.1 M and pH=6.8). The results of the study showed that a few of cancer cells and corpora amylacea do not react to lectin, although some neoplastic cells and extra cellular matrix of stroma react to lectin and alcian blue which showed the presence of sulphated and carboxylated Glycosaminoglycans. Intra nuclear reaction to lectin was shown in some epithelial cells of benign lesions. Inflammatory cells do not react to lectin in either of lesions. It seems that reaction of cells and extra cellular matrix changed in adenocarcinoma of prostate to PNA, as intranuclear reaction in some cells of benign lesions were omitted in malignant cells and substituted by apical reaction to lectin in some neoplastic cells. Future studies will probably showed the exact role of these components in course of neoplasia of organs.