Effect of isolated <i>Lactobacillus</i> from Koome (Traditional Dairy Product) on the HCT-116 colorectal carcinoma cell line
| Author | Nima Torkamani | en |
| Author | Mohammad Rahnama | en |
| Author | Narges Ahani | en |
| Author | Majid Alipour Eskandani | en |
| Orcid | Majid Alipour Eskandani [0009-0008-3521-0207] | en |
| Issued Date | 2026-07-31 | en |
| Abstract | Background: Koome is a traditional dairy product commonly consumed in rural areas of Naein city, Isfahan Province, Iran. Several studies have shown that Lactobacillus strains derived from dairy products exhibit antitumor activity by inducing apoptosis. Objectives: This study aimed to determine the potential anticancer activity of Lactobacillus-derived supernatants isolated from Koome against the growth of HCT116 colorectal cancer cells. Methods: Lactobacillus strains were isolated using MRS-based culture techniques and identified by biochemical characterization, including Gram staining, catalase testing, motility assessment, and oxidase testing. The isolated Lactobacillus species were confirmed by polymerase chain reaction (PCR). The cytotoxic effects of bacterial supernatants on HCT116 cells were evaluated using the MTT assay at different concentrations (25, 50, 100, 150, 200, and 250 μg/mL) and incubation periods (24, 48, and 72 hours). Apoptosis was assessed by flow cytometry. All experiments were performed in triplicate (n = 3), and statistical significance was analyzed using one-way ANOVA, with P < 0.05 considered statistically significant. Results: Treatment with Lactobacillus supernatants significantly reduced cell viability in a concentration- and time-dependent manner. The half-maximal inhibitory concentration (IC50) values of the Lactobacillus supernatants for HCT116 cell proliferation were 165.29 at 24 h, 150.907 at 48 h, and 130.863 at 72 h. Conclusions: Supernatants derived from Koome-isolated Lactobacillus strains suppressed proliferation and promoted apoptosis in HCT116 colorectal cancer cells compared with the control group (P < 0.05), indicating the induction of programmed cell death. These findings suggest that Lactobacillus supernatants may be promising natural candidates for further investigation as colorectal cancer therapeutics. | en |
| DOI | https://doi.org/10.5812/zjrms-169024 | en |
| URI | https://brieflands.com/journals/zjrms/articles/169024 | en |
| Keyword | Koome | en |
| Keyword | Lactobacillus | en |
| Keyword | Colorectal Cancer Cell Lines | en |
| Keyword | Flow Cytometry | en |
| Keyword | MTT | en |
| Publisher | Brieflands | en |
| Title | Effect of isolated <i>Lactobacillus</i> from Koome (Traditional Dairy Product) on the HCT-116 colorectal carcinoma cell line | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- zjrms-28-3-169024-publish-pdf.pdf
- Size:
- 558.16 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF