Anticancer effects of Perovskia abrotanoides hydroalcoholic extracts on MKN45 gastric cancer cells

Abstract

Introduction: Gastric cancer is the fourth most common cancer worldwide and the second leading cause of death from cancer. Given that existing treatments for it, but the high cost and side effects, have not demonstrated full efficacy and rely on traditional medicine and medicinal plants due to the antioxidant activity of Provskia abrotanoides, this study was performed to investigate the effects of the plant extract in improving gastric cancer. Materials and Methods: In this exprimantal study, Perovskia abrotanoides, collected and after certified specialists from the Center for Applied Science of Jahad Keshavarzi in Semnan, was extracted dry. Human gastric adenocarcinoma cell line MKN 45 was bought from the toxicity of herbal medicine on cancer cells MKN45 were investigated by using the test MTT (dimethylthiazol diphenyl tetrazolium bromide). The antioxidant properties of the hydroalcoholic extracts were evaluated by two in vitro tests, DPPH radical scavenging and reducing power (FRAP). Wound healing and morphological modification were performed in vitro to examine migration and adhesion in the gastric cancer cell line by invert microscopy. Results: The results showed that with increasing concentration of the extract of Perovskia abrotanoides, with a significant relationship, the percentage of remaining living cells of MKN45, had fallen (P˂0.001).At all concentrations of 10, 50 and 100 micrograms per ml of extract, with a significant correlation was equal with the antioxidant properties of 10 micrograms per ml vitamin C (P˂0.001). The the hydroalcoholic extracts showed considerable cytotoxic activity against cancer cell lines (IC50 = 10 μg・mL−1). It was found that the proliferation rate of MKN-45 cells decreased after treatment with the extracts in a dosedependent way. Conclusion: The results of this study showed that the Perovskia abrotanoides extract had high cytotoxic effect on gastric cancer cells (MKN45) and the percentage of lethality, with the passage of time and with increased concentration had risen.