In vitro <i>Antileishmanial</i> Effect of <i>Scrophularia</i><i>bavanatia</i> Extract on <i>Leishmania</i> (L) <i>tropica</i> [MHOM/NADIM3]
| Author | Kazem Barzegar | en |
| Author | Ali Fattahi Bafghi | en |
| Author | Ali Mohammad Ranjbar | en |
| Author | Mahmoud Vakili | en |
| Author | Seyed Jafar Adnani Sadati | en |
| Author | Tahereh Mikaeili Galeh | en |
| Author | Roghayeh Norouzi | en |
| Orcid | Kazem Barzegar [0000-0001-5766-2214] | en |
| Orcid | Ali Fattahi Bafghi [0000-0001-8469-2187] | en |
| Orcid | Ali Mohammad Ranjbar [0000-0002-2679-7041] | en |
| Orcid | Mahmoud Vakili [0000-0002-0113-7100] | en |
| Orcid | Seyed Jafar Adnani Sadati [0000-0002-5636-4626] | en |
| Orcid | Tahereh Mikaeili Galeh [0000-0002-7288-9342] | en |
| Orcid | Roghayeh Norouzi [0000-0002-4393-2380] | en |
| Issued Date | 2024-10-31 | en |
| Abstract | Background: Cutaneous Leishmaniasis (CL) is a widespread parasitic disease that causes skin lesions and ulcers. Although the World Health Organization recommends Glucantime as a treatment for CL, this remedy presents unexpected side effects and high costs. This study investigates the in vitro antileishmanial effect of Scrophulariabavanatia hydroalcoholic extract on Leishmaniatropica promastigotes. Objectives: This study aims to evaluate the in vitro antileishmanial effects of a local Scrophulariabavanatia hydroalcoholic extract on Leishmania (L.) tropica [MHOM/NADIM3] promastigotes. Methods: Various concentrations (0.2, 1, 5, 25, and 125 mg/mL) of Scrophulariabavanatia methanol extract were prepared and added to cultures containing the parasite. The amastigote form of the L. tropica strain [MHOM/IR/NADIM3] was isolated from mouse spleens and transformed into promastigotes in Novy-Nicolle-Mac Neal medium. The antileishmanial activity of Scrophulariabavanatia extract was then compared to Glucantime using cell proliferation ELISA. Results: The LC50 values in the stationary stage for both Scrophulariabavanatia extract and Glucantime (mg/mL) were greater than in the dynamic stage. The extract at concentrations of 0.2, 1, 5, 25, and 125 mg/mL did not show significant differences after 24 and 48 hours (P > 0.05) compared to negative controls but did show a significant effect after 72 hours (P = 0.000). The extract demonstrated increased effectiveness with higher concentrations and longer exposure times. Conclusions: Scrophulariabavanatia extract inhibits the survival and growth of Leishmania promastigotes. Future studies should further explore its effects on in vivo survival of the parasite. | en |
| DOI | https://doi.org/10.5812/jjhs-148933 | en |
| Keyword | Leishmania tropica | en |
| Keyword | Antileishmanial | en |
| Keyword | Scrophularia bavanatia | en |
| Keyword | Promastigotes | en |
| Keyword | in vitro | en |
| Publisher | Brieflands | en |
| Title | In vitro <i>Antileishmanial</i> Effect of <i>Scrophularia</i><i>bavanatia</i> Extract on <i>Leishmania</i> (L) <i>tropica</i> [MHOM/NADIM3] | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- jjhs-16-4-148933-publish-pdf.pdf
- Size:
- 269.14 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF