Identification of Brucella abortus (S19) immunogens using two- dimensional gel electrophoresis and westernblotting

Abstract

Background & Objectives:: Brucellosis is one of the most important zoonotic disease caused by genus of Brucella. Lipopolysaccharides (LPSs), outer membrane proteins and several ribosomal or cytosolic proteins of Brucella have been considered for diagnostic and subunit vaccine purposes. The objective of this experiment was to identify and compare immunogens of Brucella abortus(S19) in infected human and goat and immunized rabbit.