A survey on platelet surface glycoprotiens in patients with Bernard-Souliar syndrome by flowcytometry
| Author | K Shams Asanjan | en |
| Issued Date | 2005-03-31 | en |
| Abstract | Abstract Background: The gold standard diagnosis of the Bernard-Souliar syndrome (BSS), a rare disease, is to prove the absence of Ib/IX surface complex on platelets with the use of aggregometric methods. Flowcytometry is an ideal method in analysis of surface markers on cells. Objective: The use of flowcytometric analysis in diagnosis of Bernard-Souliar syndrome. Methods: 15 suspected BSS, 20 healthy persons as control group and 3 ITP patints were selected to be analysed for the presence of GPIbα and GPIIIa on the surface of platelets with the application of FITC conjugated monoclonal antibodies using flowcytometry. Findings: All healthy persons in control group and 3 ITP patients showed normal expression of both glycoprotiens on platelets using flowcytometry. All 15 suspected BSS patients showed lack of GPIbα but a normal expression of GPIIIa on platelets. Conclusion: The application of flowcytometry for diagnosis of BSS is a quick, accurate, and precise method, which together with aggregometric method can be used for diagnosis of BSS. | en |
| DOI | https://doi.org/ | en |
| Keyword | Keywords: Bernard-Souliar Syndrome | en |
| Keyword | Flowcytometry | en |
| Keyword | Glycoprteins | en |
| Keyword | Diagnosis | en |
| Publisher | Brieflands | en |
| Title | A survey on platelet surface glycoprotiens in patients with Bernard-Souliar syndrome by flowcytometry | en |
| Type | Research Article | en |
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