Detection of <italic>blaSPM-1 </italic>Metallo-?-Lactamase Gene in Imipenem-Resistant <italic>Pseudomonas aeruginosa</italic> Strains Isolated From Hospitalized Patients in Isfahan Hospitals
| Author | Mansour Sedighi | en |
| Author | Amir Hasanzadeh | en |
| Author | Saeid Safiri | en |
| Author | Naeema Syedi | en |
| Author | Shayan Mostafaei | en |
| Author | Jamshid Faghri | en |
| Issued Date | 2015-05-29 | en |
| Abstract | Background: Pseudomonas aeruginosa is an opportunistic human pathogen, which causes serious problems especially in people who have immunodeficiency. Recently, metallo-?-lactamase (MBLs) resistance in this bacterium has led to some difficulties in treating bacterial infections. The blaSPM-1 is one of the MBL gene families, which induces resistance to the carbapenem class antibiotics; this gene has not been previously assessed in Iran. Objectives: Detection and quantification of blaSPM-1- metallo-?-lactamase gene among resistant Pseudomonas aeruginosa strains (imipenem), isolated from patients in Isfahan hospitals. Patients and Methods: A total of 180 samples were isolated from various nosocomial infections. These isolates were identified as Pseudomonas aeruginosa by using biochemical tests. In order to determine their bacterial drug resistance-pattern the Kirby-Bauer disk diffusion method was utilized. Presence of MBLs in imipenem isolates was detected using the combine disk technique (IMP-EDTA). Similarly, an E-test on Mueller-Hinton agar was used to determine the minimal inhibitory concentration (MIC) of imipenem isolates. The imipenem isolates were then subjected to polymerase chain reaction (PCR) to detect the blaSPM-1 gene. Data were analyzed using the SPSS software (version 16, SPSS Inc., Chicago, IL, USA). Results: In total, 96 isolates of Pseudomonas aeruginosa were collected. Of all isolates, 34 (35.41%) were found to be imipenem-resistant P. aeruginosa. The MIC levels in all imipenem-resistant strains were MIC ? 32 ?g/mL. Thirteen (38.23%) of the imipenem-resistant P. aeruginosa isolates were MBL positive. None of the isolates carried the blaSPM-1 gene, as indicated by the PCR assay. Conclusions: The rate of imipenem resistance due to MBL has increased dramatically. Early detection and infection-control practices are the best antimicrobial strategy for this organism. | en |
| DOI | https://doi.org/10.5812/jamm.3(2)2015.26977 | en |
| Keyword | <italic>Pseudomonas aeruginosa</italic> | en |
| Keyword | Imipenem | en |
| Keyword | Nosocomial Infection | en |
| Keyword | <italic>blaSPM-1</italic> | en |
| Keyword | Metallo-?-Lactamase | en |
| Publisher | Brieflands | en |
| Title | Detection of <italic>blaSPM-1 </italic>Metallo-?-Lactamase Gene in Imipenem-Resistant <italic>Pseudomonas aeruginosa</italic> Strains Isolated From Hospitalized Patients in Isfahan Hospitals | en |
| Type | Research Article | en |
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