Histological Study of Interaction between Blastema Tissue and Decellularized Three -Dimensional Matrix of Bladder

Abstract

Background: The purpose of this study is to determine the interaction between Blastema tissue and decellularized three-dimensional matrix of ovine bladder in vitro.Materials and Methods: In order to produce relaxation of decellularized ovine bladder, combination of physiochemical methods were used. Thus, pieces of bladder were put in -4 C and then samples were put in liquid nitrogen then all samples were decellularized using chemical combination of 1% SDS (Sodium Dodecyl Sulfate). To produce Blastema tissue, a few 2 mm diameter holes were made in auricles of male New Zealand rabbits. After 72 hours, The Blastema ring was removed and it was put under sterile conditions of decellularized scaffolds inside the Blastema ring and moved to the medium. After histological stages, medium samples were stained with Hematoxylin-Eosin and Peak Indigo Carmine. The quantitative data were analyzed by ANOVA and TUKY test at the level of p