A Simple and Cost-effective Weight Drop Model to Induce Contusive Spinal Cord Injury: Functional and Histological Outcomes
| Author | Shiva Hashemizadeh | en |
| Author | Saereh Hosseindoost | en |
| Author | Khalil Pestei | en |
| Author | Mahmoudreza Hadjighassem | en |
| Orcid | Khalil Pestei [0000-0002-0538-8898] | en |
| Orcid | Mahmoudreza Hadjighassem [0000-0001-9046-2767] | en |
| Issued Date | 2022-01-31 | en |
| Abstract | Background: Animal spinal cord injury (SCI) models have provided a better perception of the mechanisms related to traumatic SCI and evaluation of the effectiveness of experimental therapeutic interventions. Objectives: The aim of this study is to develop a cost-effective modified Allen's device to induce contusive spinal cord injury. Methods: Adult male Wistar rats were subjected to contusive spinal cord injury using a customized weight drop model through 10-g weights delivered from a 25-mm height onto an exposed spinal cord. Locomotor and sensory function during 28 days were assessed. Moreover, histopathological changes were assessed at one week and 28 days post SCI. Results: All the SCI rats showed hind limb paralysis up to 48 h post SCI and neuropathic pain after injury. Histological changes similar to the previous reports for contusion model were observed. Conclusions: According to our findings, little variability was observed in the BBB score of individual rats at 28 days after injury. Our customized device to induce spinal cord injury is a simple and inexpensive alternative method to the highly sophisticated contusion device commonly used to induce SCI. | en |
| DOI | https://doi.org/10.5812/ans.118775 | en |
| Keyword | Spinal Cord Injury | en |
| Keyword | Animal Model | en |
| Keyword | Rats | en |
| Keyword | Contusion | en |
| Publisher | Brieflands | en |
| Title | A Simple and Cost-effective Weight Drop Model to Induce Contusive Spinal Cord Injury: Functional and Histological Outcomes | en |
| Type | Methods Article | en |