A Rapid Modified Antibody-Capture Enzyme-Linked Immunosorbent Assay (ELISA) for Detection HSV-1 Primary Infection in Human

Abstract

An antibody-capture enzyme-linked immunosorbent assay (ELISA) based on the reaction between anti human IgM and serum IgM and HSV-1 conjugated with horseradish peroxidase (HRP) was developed. The specificity and sensitivity of the developed HSV-1 IgM ELISA were evaluated and compared with a commercially available test kit by using sets of well defined sera of known HSV-1 primary infection. For validation, 526 serum samples have been tested. The HSV-1 IgM ELISA had acceptable sensitivity and specificity, which were comparable with that of the commercial test kit. Both assays showed some cross-reactivity with sera containing anti-HSV-2 antibodies