A Rapid Modified Antibody-Capture Enzyme-Linked Immunosorbent Assay (ELISA) for Detection HSV-1 Primary Infection in Human
| Author | M Ravanshad | en |
| Orcid | M Ravanshad [0000-0003-1808-2481] | en |
| Issued Date | 2006-04-01 | en |
| Abstract | An antibody-capture enzyme-linked immunosorbent assay (ELISA) based on the reaction between anti human IgM and serum IgM and HSV-1 conjugated with horseradish peroxidase (HRP) was developed. The specificity and sensitivity of the developed HSV-1 IgM ELISA were evaluated and compared with a commercially available test kit by using sets of well defined sera of known HSV-1 primary infection. For validation, 526 serum samples have been tested. The HSV-1 IgM ELISA had acceptable sensitivity and specificity, which were comparable with that of the commercial test kit. Both assays showed some cross-reactivity with sera containing anti-HSV-2 antibodies | en |
| DOI | https://doi.org/ | en |
| Keyword | Herpes Simplex Virus | en |
| Keyword | ELISA | en |
| Keyword | IgM | en |
| Publisher | Brieflands | en |
| Title | A Rapid Modified Antibody-Capture Enzyme-Linked Immunosorbent Assay (ELISA) for Detection HSV-1 Primary Infection in Human | en |
| Type | Research Article | en |
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