The Effects of Vitamin D3 Supplementation and Simultaneous Exercise on Aldehyde Dehydrogenase Gene Expression in Endometriosis Female Rats

Abstract

Background: Endometriosis is an injury caused by the proliferation of endometrial tissue outside the uterine cavity. Exercise and supplements may effectively remove waste materials and produce antioxidant enzymes. Objectives: The present research aimed to investigate the effects of simultaneous exercise and vitamin D3 supplement on aldehyde dehydrogenase 2 (ALDH2A1) gene expression in endometriosis female rats. Methods: The experimental method with a post-test design was used to conduct the research. The statistical population of the study consisted of three-month-old female Wistar rats. After the induction of the endometriosis model, the rats were divided into six groups. The exercise groups performed simultaneous exercises for eight weeks. Vitamin D3 supplement at 50 mg was fed to rats daily for eight weeks. The mean and standard deviation of ALDH2A values were calculated, and two-way analysis of variance (ANOVA) and Bonferroni post hoc test were investigated in female rats in different groups (P Results: Significant decreases in ALDH2A were observed by the Bonferroni test in the supplement and interval exercise groups compared to the endometriosis and endometriosis + placebo groups. Conclusions: The protocol of simultaneous exercises (strength/endurance) and vitamin D supplementation has significantly reduced aldehyde dehydrogenase 1 (ALDHA1) in endometriosis patients. Therefore, vitamin D3 supplementation with antioxidant and anti-inflammatory effects, along with sports activities, can play an important role in improving and reducing the prevalence of endometriosis.