Production and characterization of biochemical properties of L-Asparaginase by indigenous yeast isolated from soil of Iran
| Author | Hoda Nouri | en |
| Author | Hamid Moghimi | en |
| Author | Ali Khaleghian | en |
| Orcid | Ali Khaleghian [0000-0001-8520-6654] | en |
| Issued Date | 2020-03-31 | en |
| Abstract | Introduction: The use of L-asparaginase as a chemotherapeutic agent is an effective strategy for the treatment of leukemic lymphoblasts. Since the use of bacterial L-asparaginase causes side effects due to hypersensitivity reactions, search for new enzyme sources is one of the active research areas. In this study, the characterization of produced L-asparaginase by indigenous yeast strains was investigated. Material and methods: In this study, 130 yeast strains were evaluated. Remarkably, AG90 isolate with 0.94 IU/ml enzyme production was selected as the superior strain. Results: The molecular identification of the AG90 showed that this strain exhibited a high level of similarity (99%) with Sarocladium sp. and introduced as a Sarocladium sp. AG90. The fermentation broth of the superior strain showed that 86% of the enzyme was produced extracellularly. The Km and Vmax values of the produced enzyme for the L-Asparagine substrate were calculated as 9.74 mM and 19.19 μmol/min, respectively. Discussion: The obtained results in this study can help to introduce a new enzyme with eukaryotic origin to reduce the toxicity, sensitivity and side effects associated of drug consumption. | en |
| DOI | https://doi.org/ | en |
| Keyword | Asparaginase | en |
| Keyword | Yeast | en |
| Keyword | Leukemia | en |
| Keyword | Iran | en |
| Keyword | آسپاراژیناز | en |
| Keyword | مخمر | en |
| Keyword | سرطان خون | en |
| Keyword | ایران | en |
| Publisher | Brieflands | en |
| Title | Production and characterization of biochemical properties of L-Asparaginase by indigenous yeast isolated from soil of Iran | en |
| Type | Research Article | en |