In Vitro Evaluation of the Biocompatibility and Stability of Fluorescent Dil Dye on Mouse Embryonic Fibroblast Cells
| Author | Marzyeh Haghshenas | en |
| Author | Elham Hoveizi | en |
| Author | Tayebeh Mohammadi | en |
| Author | Seyed Reza Kazemi Nezhad | en |
| Orcid | Elham Hoveizi [0000-0002-8909-5004] | en |
| Orcid | Seyed Reza Kazemi Nezhad [0000-0003-2919-1915] | en |
| Issued Date | 2019-01-31 | en |
| Abstract | Background: There are few methods for cell labeling in basic studies of cell-migration, tissue engineering, and cell therapy. Recent progress in cell trackers has led to an increasing number of clinical trials involving the treatment of various diseases based on cell transplantation. Objectives: In this study, we investigated the effects of different concentrations of DiI as a cell labeling dye on the viability and proliferation of mouse embryonic fibroblasts (MEFs). Methods: MEFs were divided into two groups of control and experimental (treated with Dil dye). We treated MEFs with three concentrations of the dye and after 1, 5, 7 and 14 days, these cells were analyzed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and 4’, 6-diamidino-2-phenylindole (DAPI) staining. Also, the stability of the dye in treated MEFs was examined by immunofluorescence. Results: Our results showed that cell viability was significantly different between the groups that received Dil at the concentrations of 1 and 2 µg/mL and the control group. However, cell viability was not significantly different between the group that received Dil (0.5 µg/mL) and the control group. Conclusions: Therefore, Dil at concentrations higher than 0.5 µg/mL was toxic for MEFs. We suggest that Dil dye, which is concentration-dependent, can be used as a safe and stable fluorescent dye for cell labeling both in-vivo and in-vitro. | en |
| DOI | https://doi.org/10.5812/gct.85521 | en |
| Keyword | Cell Viability | en |
| Keyword | Stem Cells | en |
| Keyword | Cell Therapy | en |
| Keyword | Cell Tracker | en |
| Keyword | Dil Dye | en |
| Publisher | Brieflands | en |
| Title | In Vitro Evaluation of the Biocompatibility and Stability of Fluorescent Dil Dye on Mouse Embryonic Fibroblast Cells | en |
| Type | Research Article | en |