Antigenicity of recombinant L7/L12 in patients with brucellosis
| Author | Hamid Abtahi | en |
| Author | Ali hatef Salmanian | en |
| Orcid | Hamid Abtahi [0000-0002-3247-3289] | en |
| Issued Date | 2012-03-31 | en |
| Abstract | Introduction: Immune response to recombinant L7/L12, in addition to protective role, may show its importance in detection Brucellosis tests. The aim of this study was to examine antigenicity of recombinant L7/L12 from Brucella abortus by Brucellosis human sera. Material and Methods : We amplified L7/L12 gene by polymerase chain reaction (PCR) method and sub- cloned to prokaryotic expression vector pET28a. Escherichia coli BL21-DE3-plySs was transformed with pET28a-L7/L12 and gene expression was induced by IPTG. Recombinant L7/L12 was further analyzed by Western Blot. Sera reactivity of five infected individual were further analyzed against the recombinant L7/L12 protein. Results: The sequencing result was confirmed by Sanger method and it was the same as L7/L12 gene. Escherichia coli BL21 (DE3) pLysS was transformed with pET28a-L7/L12 and gene expression was induced by IPTG. The expressed protein was purified by affinity chromatography by Ni-NTA resin. The data also indicated that L7/L12 protein from Brucella abortus recognized by patient sera. Conclusion: Our data showed that recombinant L7/L12 protein can be produced by pET28a in Escherichia coli. This protein was recognized by sera in infected human as an antigen. Therefore, recombinant L7/L12 has same epitopes with natural form of this antigen. Recombinant L7/L12 also seems to be a promising antigen for protection and serologic diagnosis of Human brucellosis. | en |
| DOI | https://doi.org/ | en |
| Keyword | Brucella abortus, L7/L12 recombinant protein, Brucellosis, Blotting, Western, Escherichia coli | en |
| Keyword | بروسلا آبورتوس، پروتئین نو ترکیب L7/L12، تب مالت، وسترن بلات ، اشریشیاکولی | en |
| Publisher | Brieflands | en |
| Title | Antigenicity of recombinant L7/L12 in patients with brucellosis | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- article-1-1144-fa.pdf.pdf
- Size:
- 315.16 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF