Isolation and identification of mesenchymal and neural crest characteristics of dental pulp derived stem cells
| Author | nazila niapour | en |
| Author | zahra tagipour | en |
| Author | hossein salehi | en |
| Author | abolfazl bagheri | en |
| Author | anahita rahani | en |
| Author | mahdie talebi | en |
| Author | nowrouz najafzadeh | en |
| Author | ali niapour | en |
| Issued Date | 2015-09-30 | en |
| Abstract | Introduction: Mesenchymal stem cells (MSC) are considered as a unique source of adult stem cells which are usually accessible with minimum ethical concerns. MSCs can be used in stem cell biology , different to other cells, tissue engineering, regenerative medicine and future of cancer treatment researches. The aim of the present study was to isolate dental pulp derived stem cells (DPSCs) , characterizing them with common techniques and determining neural crest gene s expression profile. Materials and Methods: After collecting the teeth , the pulp chamber exposed and the pulp tissue digested with collagenase IV. Dental pulp derived cells were resuspended and incubated in alpha modified minimum essential medium eagle ( αMEM ) supplemented with 15 % fetal bovine serum at 37°C temperature, 95% humidity and 5% CO2 . The osteogenic and adipogenic differentiation potentials for dental pulp derived cells were assessed in appropriate conditions. Flow-cytometry analyses were utilized to evaluate the percentage of CD90 and CD45 positive markers within isolated cells . The expression of p75ngfr, slug and sox10 genes was measured via RT-PCR. Results: Dental pulp derived cells were appropriately attached to the culture vessels and showed high proliferation rate on culture medium . Cells could also differentiate to osteogenic and adipose cells displayed by Alizarin red and Oil red staining . Higher percentage of pulp derived cells were CD90 positive , while the percentage of CD45 expression, as a marker of hematopoietic cells, was negligible . Furthermore , the RT-PCR also revealed the expression of p75ngfr, slug and sox10 in the MSCs isolated from the dental pulps. Conclusion: Dental pulp derived cell have the mesenchymal stem cells characteristics and at least a portion of them have neural crest origin and identity | en |
| DOI | https://doi.org/ | en |
| Keyword | Mesenchymal Stromal cells | en |
| Keyword | Dental pulp | en |
| Keyword | Cell differentiation | en |
| Keyword | Neural Crest | en |
| Keyword | سلولهای بنیادی مزانشیمی | en |
| Keyword | پالپ دندان | en |
| Keyword | تمایز سلولی | en |
| Keyword | ستیغ عصبی | en |
| Publisher | Brieflands | en |
| Title | Isolation and identification of mesenchymal and neural crest characteristics of dental pulp derived stem cells | en |
| Type | Research Article | en |
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