Detection and cloning of desulfurization operon sox(dszAB) in rhodococcus FMF native bacterium

Abstract

Introduction: Native Rhodococcus FMF bacterium was selected among several isolated Iranian bacterium. Primary studies have shown that the bacterium use dibenzothiophene as a sulfur source. Since desulfurization is taken by a biochemical pathway, the present project was designed to detect, clone and sequence of desulfurization genes of the bacterium. Materials and Methods: Desulfurization operon was isolated from Rhodococcus erythropolis IGTS8 and was used as a probe for detection of desulfurization 4S pathway genes by southern blotting technique. Specific primers were designed and the dszA and B genes were amplified by polymerase chain reaction (PCR). PCR products were recovered from agarose gel and were cloned into the PTZ57R vector. Results: In this study, recombinant PTZAB57R vector was constructed by the insertion of dszA and B genes into the PTZ57R vector. The clones were confirmed by restriction digestion analysis. The PTZAB57R was extracted by large-scale method and was sequenced (MWG DNA Biotech). Conclusion: Comparison of sequences of dszA and B genes from native Rhodococcus FMF bacterium with Rhodococcus sp. IGTS8 bacterium were shown complete identity between them, which show that desulfurization pathway is conserved in this bacterium.