Study of Effects of Sex and Body weight on Basal Acid Secretion in Rat

Abstract

The amount of gastric acid secretion is not the same and changes during the time. May of the sex hormone effect on gastric acid secretion, all attempted were to use male animal in experiments. Because of different reports on basal acid output in rat (10 to 70 μmol/h), in present study was attempted to evaluate the effect of sex and body weight on basal acid output. There was rats (n=51) range weight 140-370 g male and female was kept in temperature (23 ± 2 C) and light period. (12h light, 12h dark) Experiments were started in 8:00 AM, and 18 hours before experiment, animal were starved from food (except water). Animal’s anesthetized with Sodium thiopental, and a silicon tube was inserted in stomach via duodenum. The amount of acid was measured with aid of automatic acid titrator (TTT 80), with Na OH 0.1 N to pH=7. Using student t-Test and Spearman correlation coefficient compared the results (P