Antimicrobial Potential of <i>Xenorhabdus khoisanae</i> Kamangar, 2024 from the Digestive Tract of Wild Worker Honeybee, <i>Apis florea </i>
| Author | Fatemeh Kamangar Zade | en |
| Author | Neda Aminijam | en |
| Author | Abbas Moridnia | en |
| Author | Maryam Gheibipour | en |
| Author | Mohammad Amin Behmanesh | en |
| Author | Babak Elyasi Far | en |
| Orcid | Abbas Moridnia [0000-0002-8393-839X] | en |
| Orcid | Mohammad Amin Behmanesh [0000-0002-4292-5381] | en |
| Orcid | Babak Elyasi Far [0000-0003-2209-3095] | en |
| Issued Date | 2024-08-31 | en |
| Abstract | Background: The discovery of novel antimicrobial compounds is crucial for addressing the global challenge of antibiotic-resistant pathogens. Natural products derived from bacteria remain a key source of new antimicrobial agents. The gut microbiome of wild honeybees contains rare bacterial species with antibacterial properties that may offer valuable medical applications. Methods: The intestinal contents of adult worker honeybees were collected and enriched using tryptic soy broth (TSB) medium. The supernatants of broth cultures were spread on tryptic soy agar (TSA) plates to isolate diverse colonies. The antibacterial potential of the isolates was assessed using the disk diffusion method, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for superior isolates. Fermented broth cultures underwent extraction and purification of antimicrobial compounds, followed by gas chromatography-mass spectrometry (GC-MS) analysis. Isolates that effectively suppressed human pathogens were identified through 16S rRNA gene sequencing. Results: Based on the results, nine microorganisms were isolated from the intestinal contents of adult worker honeybees. Among them, Xenorhabdus khoisanae strain Kamangar, 2024 demonstrated the strongest antibacterial activity, with a mean inhibition zone of 29 ± 1.2 mm against Staphylococcus aureus (n = 3). This strain also exhibited antimicrobial activity against Streptococcus mutans, Enterococcus faecalis, Klebsiella pneumoniae, Shigella flexneri, Pseudomonas aeruginosa, and Candida albicans. Both the MIC and MBC values against S. aureus were 0.011 mg/mL. The GC-MS analysis identified indole as the primary active compound, known for its diverse pharmacological properties. Conclusions: Xenorhabdus khoisanae Kamangar, 2024 demonstrates potent antimicrobial activity, making it a promising candidate for the development of novel antibiotics and alternative therapies targeting drug-resistant pathogens. Its antimicrobial compounds may contribute to resistance mitigation and have potential pharmaceutical and industrial applications. | en |
| DOI | https://doi.org/10.5812/jai-162702 | en |
| Keyword | Honeybees | en |
| Keyword | <i>Xenorhabdus khoisanae</i> Kamangar, 2024 | en |
| Keyword | Indole | en |
| Keyword | Human Pathogenic Bacteria | en |
| Publisher | Brieflands | en |
| Title | Antimicrobial Potential of <i>Xenorhabdus khoisanae</i> Kamangar, 2024 from the Digestive Tract of Wild Worker Honeybee, <i>Apis florea </i> | en |
| Type | Research Article | en |