Anti-Hemorrhagic Effect of Horsetail, Ortie, Alfalfa, Chêne, and Aleppo oakin an Experimental Model of Rats - a Potential Theoretic Approach for Traumatic Bleeding

Abstract

Prompt bleeding control in civil accidents, incidents, and combat casualties is critically essential. Preparing efficient, portable, and low-cost local anti-hemorrhagic products with minimum side effects is one of the main challenges of using them in hemorrhage control. Anti-hemorrhagic effect of some medicinal plants, including Horsetail (H), Ortie (O), Alfalfa (A1), Chêne (C), and Aleppo oak (A2), were evaluated in the femoral arterial bleeding rat model. Materials and Methods: After plant extraction by the maceration method, forty male rats received general anesthesia, and the left femoral artery was surgically transected. Bleeding was treated with direct gauze pressure, in both the control (without treatment) and test groups added with the mixture of five herbal extracts at 200 g/l concentration (M-200), the mixture of five herbal extracts at 400 g/l concentration (M-400), and individual extracts at 400 g/l concentration). Bleeding stoppage time (BST), blood loss volume (BLV) was defined and some blood coagulation tests were assessed. Results: There was no statistically significant difference of BLV between mix-200 and control groups, though it was significantly lower for mix-400 than that the control and mix-200 groups (P