Effect of CD44 Aptamer on Snail Metastasis Factor and Aggressiveness of MDA-MB-231 Breast Cancer Cell Line
| Author | Soudabeh Kavousipour | en |
| Author | Pooneh Mokarram | en |
| Author | Mahdi Barazesh | en |
| Author | Elham Arabizadeh | en |
| Author | Vahid Razban | en |
| Author | Zohreh Mostafavipour | en |
| Author | Shiva Mohammadi | en |
| Author | Samira Sadat Abolmaali | en |
| Orcid | Soudabeh Kavousipour [0000-0001-9724-9942] | en |
| Orcid | Pooneh Mokarram [0000-0002-9717-0473] | en |
| Orcid | Vahid Razban [0000-0002-8966-6081] | en |
| Orcid | Zohreh Mostafavipour [0000-0002-3779-177X] | en |
| Issued Date | 2020-03-28 | en |
| Abstract | Background: The intracellular signaling pathways stimulated by CD44/hyaluronic acid (HA) interaction play a central role in the invasion and migration of cancer cells. Epithelial-mesenchyme transition (EMT) is an important factor in cancer metastasis and migration, which can be stimulated by the snail transcription factor. Previous studies showed cells that were subjected to snail-induced EMT, characterized by a CD44high/CD24low phenotype, expressed at their surface. Objectives: The aim of this study was to assess the inhibitory effect of CD44/HA interaction on the snail expression and invasive behavior of aggressive breast cancer cell line with a high CD44 expression in 2D and 3D culture. The cell surface binding capacity of the selected aptamer was evaluated via flow cytometry assay. Methods: To test our hypothesis, we disrupted the CD44/HA interaction by DNA aptamer, which specifically binds to the Hyaluronic Acid Binding Domain (HABD) of CD44. Then, expression level of snail mRNA was evaluated in MDA-MB 231 cells, cultured in 2D and 3D conditions by real-time PCR. Furthermore, invasive behavior was evaluated, using wound healing assay. Results: The results of this study showed that CD44 aptamer reduced snail expression and invasive behavior in MDA-MB 231 cell line. In addition, our result indicated that cells cultured in 3D were more sensitive to the aptamer in comparison to those cultured in the 2D model. Conclusions: The inhibition of CD44-HA interaction, using aptamer, negatively regulates the CD44 function in aggressive breast cancer cell line with the high level of CD44 expression. | en |
| DOI | https://doi.org/10.5812/semj.94641 | en |
| Keyword | Aptamer | en |
| Keyword | Breast Cancer | en |
| Keyword | CD44 | en |
| Keyword | 3D Culture | en |
| Keyword | Metastasis | en |
| Publisher | Brieflands | en |
| Title | Effect of CD44 Aptamer on Snail Metastasis Factor and Aggressiveness of MDA-MB-231 Breast Cancer Cell Line | en |
| Type | Research Article | en |