Human Rhinovirus Molecular Epidemiology and Genotyping in Iranian Military Trainees with Acute Respiratory Symptoms

AuthorFahimeh Safarnezhad Tameshkelen
AuthorAli Salimi Jedaen
AuthorAhmad Tavakolien
AuthorMohammad Hadi Karbalaie Niyaen
AuthorMorteza Izadien
AuthorFarhad Zamanien
AuthorHossein Keyvanien
OrcidFahimeh Safarnezhad Tameshkel [0000-0001-8953-5447]en
OrcidAli Salimi Jeda [0000-0003-3197-9509]en
OrcidAhmad Tavakoli [0000-0003-1857-0610]en
OrcidMohammad Hadi Karbalaie Niya [0000-0002-1364-5743]en
OrcidMorteza Izadi [0000-0002-5412-4426]en
OrcidFarhad Zamani [0000-0001-7409-3412]en
Issued Date2020-12-31en
AbstractBackground: Human rhinovirus (HRV) is still the most prevalent viral infection in humans and a significant cause of acute respiratory tract infections (ARTIs) in many communities, including military personnel undergoing basic training. Objectives: In this research, we assessed the molecular epidemiology, genotyping, and phylogenetic classification of HRVs in Iranian military trainees with respiratory infections (RI). Methods: For HRV identification and genotyping, respiratory specimens were obtained, and RT-PCR was conducted for genotyping and phylogenetic analysis of HRV utilizing primers for the 5-UTR region. Results: Among 400 Iranian military trainees (average age of 21 ± 4 years, the range of 18 - 57 years) with respiratory infections, HRV was detected in 29 patients (7%) using RT-PCR. The direct sequencing of PCR products from 10 specimens showed that the incidence of type A (n = 5, 50%) was higher than that of type B (n = 4, 40%) and type C (n = 1, 10%). There were no significant associations between HRV and respiratory and clinical symptoms, blood group, and indoor or outdoor conditions (P-value > 0.05). Conclusions: This research was the first to record HRV as a significant cause of respiratory problems among military trainees in Iran, with a frequency of 7%. The most prevalent genotype was HRV-A, which may be applicable in epidemiological and clinical studies, as well as vaccination plans.en
DOIhttps://doi.org/10.5812/jamm.111224en
URIhttps://brieflands.com/journals/jamm/articles/111224en
KeywordHuman Rhinovirusen
KeywordRespiratory Tract Infectionen
KeywordMilitary Traineesen
KeywordGenotypingen
PublisherBrieflandsen
TitleHuman Rhinovirus Molecular Epidemiology and Genotyping in Iranian Military Trainees with Acute Respiratory Symptomsen
TypeResearch Articleen

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