Cancer cell detection using electrochemical nanobiosensor based on graphene / gold nanoparticle

Abstract

Introduction: Nowadays, early cancer detection and effective treatment is crucial for improved prognosis and cancer management. In particular, the accurate qualitative detection of cancer cells represents a critical step in cancer diagnosis. The aim of this study was to examine Cancer cell detection using electrochemical nanobiosensor based on graphene / gold nanoparticle. Materials and Methods: Modified graphene oxide/gold nanoparticle electrodes were employed to increase the sensitivity of human breast cancer MCF-7 cells detection, using CD44 biomarker. Frist the electrodes were modified with graphene, then gold nanoparticles were sediment on graphene-modified electrode. Then CD44 monoclonal antibody conjugated on the surface of gold nanoparticles, on graphene-modified electrode. Finally, the performance of the fabricated biosensors were investigated by using a common reference electrode composed of silver-silver chloride and a common platinum counter electrode at different antigen concentrations with the buffer and serum. Results: The proposed electrochemical cytosensor delivered a high sensitivity with the average of 1.12 μA / cells ml-1, and a low detection limit of 6 cells. Conclusion: These results indicate that the cytosensor has great potential in diagnosis of cancers