In vitro Biological Evaluation of a Gelatin-Alginate-Based Hydrogel Loaded With Silver Sulfadiazine for Wound Care
| Author | Hadi Hossainpour | en |
| Author | Hosna Alvandi | en |
| Author | Soheila Zare | en |
| Author | Faranak Aghaz | en |
| Author | Tahereh Naseriyeh | en |
| Author | Elham Arkan | en |
| Author | Ramin Abiri | en |
| Author | Amirhooshang Alvandi | en |
| Orcid | Hadi Hossainpour [0000-0002-3784-2645] | en |
| Orcid | Ramin Abiri [0000-0002-4657-2413] | en |
| Orcid | Amirhooshang Alvandi [0000-0002-4963-2485] | en |
| Issued Date | 2026-05-31 | en |
| Abstract | Background: Silver sulfadiazine (SSD) is widely used to treat burn wound infections; however, conventional SSD creams require frequent reapplication and may cause pain during dressing removal. Objectives: This study aimed to develop and evaluate a gelatin/alginate (GEL/ALG) hydrogel loaded with 1% SSD as a nonadherent, antimicrobial wound dressing. Methods: The hydrogel was prepared by mixing GEL and ALG at an 80:20 ratio, incorporating SSD, and cross-linking the matrix with calcium chloride. Results: The hydrogel exhibited a white, uniform, crack-free appearance and complete flexibility. Scanning electron microscopy revealed a porous three-dimensional structure suitable for fluid absorption and drug release. Fourier-transform infrared spectroscopy confirmed that SSD was not chemically bound to the polymer matrix. The hydrogel demonstrated strong antibacterial activity against Staphylococcus aureus (21-mm inhibition zone) and Acinetobacter baumannii (20 mm), comparable to that of gentamicin (20 μg/disk), and moderate activity against Pseudomonas aeruginosa (10 mm). Antifungal testing against Candida albicans produced a 12-mm inhibition zone. The material showed high swelling capacity, favorable drug-release kinetics, and mechanical strength suitable for use at various body sites. Hemocompatibility testing showed hemolysis below 5%, confirming safety for wound application. Conclusions: The GEL/ALG/SSD hydrogel functions as a nonadherent dressing by maintaining a moist gel layer without adhering to the wound or a secondary bandage. These findings support this hydrogel as a promising candidate for advanced wound care, providing antimicrobial activity, exudate management, and pain-free removal within a single dressing system. | en |
| DOI | https://doi.org/10.5812/jjm-170957 | en |
| URI | https://brieflands.com/journals/jjm/articles/170957 | en |
| Keyword | Alginate | en |
| Keyword | Gelatin | en |
| Keyword | Hydrogels | en |
| Keyword | Silver Sulfadiazine | en |
| Keyword | Wound Healing | en |
| Publisher | Brieflands | en |
| Title | In vitro Biological Evaluation of a Gelatin-Alginate-Based Hydrogel Loaded With Silver Sulfadiazine for Wound Care | en |
| Type | Research Article | en |