The Effect of Intrathecal Injection of Dextromethorphan on the Experimental Neuropathic Pain Model
| Author | Achmad Fahmi | en |
| Author | Yunus Kuntawi Aji | en |
| Author | Dirga Rachmad Aprianto | en |
| Author | Akbar Wido | en |
| Author | Asadullah Asadullah | en |
| Author | Nurkholis Roufi | en |
| Author | Danti Nur Indiastuti | en |
| Author | Heri Subianto | en |
| Author | Agus Turchan | en |
| Orcid | Achmad Fahmi [0000-0003-0464-5482] | en |
| Orcid | Yunus Kuntawi Aji [0000-0002-1877-6312] | en |
| Orcid | Dirga Rachmad Aprianto [0000-0003-2591-1938] | en |
| Orcid | Asadullah Asadullah [0000-0002-9368-4717] | en |
| Issued Date | 2021-06-30 | en |
| Abstract | Background: Peripheral glucocorticoid receptors (GRs) are altered by peripheral nerve injury and may modulate the development of neuropathic pain. Two central pathogenic mechanisms underlying neuropathic pain are neuroinflammation and N-methyl-D-aspartate receptor (NMDAR)-dependent neural plasticity in the spinal cord. Objectives: This study examined the effect of the non-competitive NMDAR antagonist dextromethorphan on partial sciatic nerve ligation (PSL)-induced neuropathic pain and the spinal expression of the glucocorticoid receptor (GR). Methods: Male mice were randomly assigned into a sham group and two groups receiving PSL followed by intrathecal saline vehicle or dextromethorphan (iDMP). Vehicle or iDMP was administered 8 - 14 days after PSL. The hotplate paw-withdrawal latency was considered to measure thermal pain sensitivity. The spinal cord was then sectioned and immunostained for GR. Results: Thermal hyperalgesia developed similarly in the vehicle and iDMP groups prior to the injections (P = 0.828 and 0.643); however, it was completely mitigated during the iDMP treatment (P < 0.001). GR expression was significantly higher in the vehicle group (55.64 ± 4.50) than in the other groups (P < 0.001). The iDMP group (9.99 ± 0.66) showed significantly higher GR expression than the sham group (6.30 ± 1.96) (P = 0.043). Conclusions: The suppression of PLS-induced thermal hyperalgesia by iDMP is associated with the downregulation of GR in the spinal cord, suggesting that this analgesic effect is mediated by inhibiting GR-regulated neuroinflammation. | en |
| DOI | https://doi.org/10.5812/aapm.114318 | en |
| Keyword | Neuropathic Pain | en |
| Keyword | Intrathecal | en |
| Keyword | Glucocorticoid Receptor | en |
| Keyword | Dextromethorphan Hydrobromide | en |
| Publisher | Brieflands | en |
| Title | The Effect of Intrathecal Injection of Dextromethorphan on the Experimental Neuropathic Pain Model | en |
| Type | Research Article | en |