Analysis of Hotspot <i>PIK3CA</i> Mutations (E542K and E545K) in Iranian Colorectal Cancer Patients
| Author | Mohammad Amin Behmanesh | en |
| Author | Zahra Masouri | en |
| Author | Mohammad Reza Abyaz | en |
| Author | Habib Habibimoghadam | en |
| Author | Abbas Moridnia | en |
| Orcid | Mohammad Amin Behmanesh [0000-0002-4292-5381] | en |
| Orcid | Mohammad Reza Abyaz [0000-0002-6138-2554] | en |
| Orcid | Abbas Moridnia [0000-0002-8393-839X] | en |
| Issued Date | 2024-11-30 | en |
| Abstract | Background: Colorectal cancer (CRC) is the third most common and the second deadliest cancer, causing about 10% of cancer deaths worldwide. Changes in the PIK3CA gene are important for CRC development and treatment response. Objectives: This study aimed to evaluate mutations in the PIK3CA gene, specifically E542K and E545K, linked to CRC in the Iranian population. Methods: This study analyzed 39 CRC tumor samples from Dr. Ganjavian Hospital and Imam Ali Clinic in Dezful. DNA was extracted using the phenol-chloroform method, followed by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) and sequencing to identify mutations. Results: Thirty-nine patients were examined in this study, comprising 14 women (35.9%) and 25 men (64.1%). The mean age of the participants was 59.2 years, with a standard deviation of 15.9 years. The findings indicated that 46.1% of the patients presented with tumors exceeding 4.5 cm in size, whereas 53.9% had tumors that were 4.5 cm or smaller. Lymphoid metastasis was observed in 55.81% of all patients, with those having tumors larger than 4.5 cm exhibiting a notably higher incidence of this metastasis. The most frequently encountered tumor grades among the participants were grades 1 and 2. Staging was identified as follows: 18.42% were classified as stage 1, 50% as stage 2, and 31.58% as stage 3. No E542K or E545K mutations were detected in any of the 39 CRC patients (0%). Conclusions: This research suggests no correlation between mutations in the PIK3CA gene and the occurrence of CRC within the studied population, but a significant correlation was observed between tumor size > 4.5 cm and lymphoid metastasis (P < 0.001). These findings underscore the importance of population-specific genetic profiling in CRC, even in the absence of expected mutations. | en |
| DOI | https://doi.org/10.5812/jai-162972 | en |
| URI | https://brieflands.com/journals/jai/articles/162972 | en |
| Keyword | <i>PIK3CA</i> | en |
| Keyword | Colorectal Cancer | en |
| Keyword | Mutation | en |
| Keyword | Iran | en |
| Publisher | Brieflands | en |
| Title | Analysis of Hotspot <i>PIK3CA</i> Mutations (E542K and E545K) in Iranian Colorectal Cancer Patients | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- jai-4-4-162972-publish-pdf.pdf
- Size:
- 336.52 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF