Differentiation of Periodontal Ligament Stem Cells Into Osteoblasts on Hybrid Alginate/ Polyvinyl Alcohol/ Hydroxyapatite Nanofibrous Scaffolds
| Author | Naghmeh Bahrami | en |
| Author | Mohammad Bayat | en |
| Author | Armin Ai | en |
| Author | Mehdi Khanmohammadi | en |
| Author | Jafar Ai | en |
| Author | Akbar Ahmadi | en |
| Author | Majid Salehi | en |
| Author | Somayeh Ebrahimi-Barough | en |
| Author | Arash Goodarzi | en |
| Author | Roya Karimi | en |
| Author | Abdolreza Mohamadnia | en |
| Author | Azam Rahimi | en |
| Orcid | Naghmeh Bahrami [0000-0002-3575-4094] | en |
| Orcid | Majid Salehi [0000-0002-0974-401X] | en |
| Issued Date | 2018-10-31 | en |
| Abstract | The efficacy of Alginate/ Polyvinyl alcohol/ Hydroxyapatite nanopowders (Alg/PVA/HANPs) mats was investigated for increasing periodontal ligament stem cells (PDLSCs) differentiation into the osteoblast cells. The Alg/PVA/HANPs nanofibers were fabricated through electospinning process and following ionic crosslinking of Ca+2 and glutaraldehyde crosslinkers. The physical features of mats and expression of osteoblast cell markers were evaluated. Results showed the diameter of nanofiber increases with decreasing the incorporation of HANPs in Alg/PVA nanofiber. According to MTT assay the viability of cells on scaffolds are better in the all of groups, so the scaffolds were not toxic for the cultured cells. The osteogenic culture media appropriately enhanced osteopontin and osteocalcin markers. Furthermore, in vitro study indicated that the Alg/PVA/HANPs nanofiber provided a suitable three-dimensional structure for osteoblast cells differentiation. Thus, the Alg/PVA/HANPs scaffold can be recommended for in bone repair. | en |
| DOI | https://doi.org/10.5812/ans.74267 | en |
| Keyword | Periodontal Ligament Stem Cells | en |
| Keyword | Alginate/ Polyvinyl Alcohol/ Hydroxyapatite | en |
| Keyword | Osteoblast Cells | en |
| Keyword | Nanofiber Scaffold | en |
| Publisher | Brieflands | en |
| Title | Differentiation of Periodontal Ligament Stem Cells Into Osteoblasts on Hybrid Alginate/ Polyvinyl Alcohol/ Hydroxyapatite Nanofibrous Scaffolds | en |
| Type | Research Article | en |