Anti-Proliferative and Apoptotic Effects of Beta-Ionone in Human Leukemia Cell Line K562
| Author | Zohreh Faezizadeh | en |
| Author | Amir Gharib | en |
| Author | Masoud Godarzee | en |
| Orcid | Zohreh Faezizadeh [0000-0002-7537-0394] | en |
| Issued Date | 2016-06-30 | en |
| Abstract | Background: Beta-ionone is an aroma compound found in the Rosaceae family. Some evidence supported that beta-ionone has a great potential for cancer prevention. To date, the anti-proliferative and apoptotic effects of beta-ionone in human leukemia cell line K562 were not studied. Objectives: Hence, we investigated whether beta-ionone could inhibit cell growth and induce apoptosis in the K562 cells. Materials and Methods: In this experimental study, human leukemia cell line K562 was cultured and anti-proliferation effect of beta-ionone with different doses (25 - 400 µm) at different times (24 - 96 hours) on treated cells was evaluated by the MTT assay. To determine apoptosis rate, the Hoechst 33342 staining and flow cytometry was performed. Results: The MTT assay showed that beta-ionone inhibited proliferation of K562 cells in a dose-dependent manner significantly (P = 0.0008). Moreover, the increased apoptotic rate was found after incubation of K562 cells with 200 µm beta-ionone. The Hoechst staining and flow cytometry analysis indicated that beta-ionone could increase apoptosis of K562 cells in a dose-dependent manner. Conclusions: The results demonstrated that beta-ionone has anti-proliferative and apoptotic effects on K562 cells, and in the future may be used in the treatment of some leukemia sub-types. | en |
| DOI | https://doi.org/10.17795/zjrms-7364 | en |
| Keyword | Beta-Ionone | en |
| Keyword | K562 Cell Line | en |
| Keyword | MTT Assay | en |
| Keyword | Apoptosis | en |
| Publisher | Brieflands | en |
| Title | Anti-Proliferative and Apoptotic Effects of Beta-Ionone in Human Leukemia Cell Line K562 | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- zjrms-18-06-7364.pdf
- Size:
- 948.53 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF