Standardization of Molecular Diagnostic of the entC and ent E <i>Staphylococcus aureus </i>Isolated from Human Infections in Zabol
| Author | Khadije Rezaie Keikhaie | en |
| Author | Gholamreza Bagheri | en |
| Author | Atefeh Kamali | en |
| Author | Saphora Bazi | en |
| Author | Zahra Shahi | en |
| Author | Fereshteh Javadian | en |
| Issued Date | 2017-10-31 | en |
| Abstract | Background: Staphylococcus aureus is one of the most important causes of hospital infections around the world. It has been found in various studies, that 15% - 18% of the Staphylococcus aureus strains isolated from different sources are capable of producing enterotoxin. The aim of this study is identifying Staphylococcus aureus, types C and E, which produce Enterotoxin, from human Infections in Zabol by PCR. Methods: The present study was conducted on 60 urine samples in a 1-year period. Samples were randomly collected from different parts of the city of Zabol and transferred to the university lab in a minimum period of time by maintaining cold conditions. DNA extraction was performed and PCR was done for enterotoxin E and C. Results: The results of this study showed that isolated S. aureus was resistant to antibiotics including cefazolin (7.3%), doxycycline (12.10%), erythromycin (22.3%), rifampin (9.7%), ciprofloxacin (18.10%), and Co-trimoxazole (14.5%). The results of this study showed that the prevalence of enterotoxin E gene in S. aureus samples was 1.5%. Conclusions: Regarding the importance of S. aureus secretes, 2 types of toxins with super antigen activity, enterotoxins, of which there are 6 antigenic types (named SE-A, B, C, D, E and G). In case of expression of enterotoxin genes, rapid treatment of the infection seems to be necessary. | en |
| DOI | https://doi.org/10.5812/gct.59214 | en |
| Keyword | Enterotoxin | en |
| Keyword | PCR | en |
| Keyword | <i>Staphylococcus aureus</i> | en |
| Publisher | Brieflands | en |
| Title | Standardization of Molecular Diagnostic of the entC and ent E <i>Staphylococcus aureus </i>Isolated from Human Infections in Zabol | en |
| Type | Research Article | en |
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