Dendrosomal Nano-Curcumin Modulates P-Glycoprotein Activity and Induces Apoptosis in Wild Type and P53-Mutant Breast Cancer Cell Lines
| Author | Aref Sobhkhizi | en |
| Author | Esmaeil Babaei | en |
| Author | Hewa Jalal Azeez | en |
| Author | Farzad Katiraee | en |
| Author | Bashdar Mahmud Hussen | en |
| Author | Mohammad Ali Hoseinpour Feizi | en |
| Orcid | Esmaeil Babaei [0000-0002-1603-5166] | en |
| Orcid | Hewa Jalal Azeez [0000-0002-2513-8263] | en |
| Orcid | Farzad Katiraee [0000-0002-8634-4378] | en |
| Orcid | Bashdar Mahmud Hussen [0000-0002-7647-0119] | en |
| Orcid | Mohammad Ali Hoseinpour Feizi [0000-0002-1508-5022] | en |
| Issued Date | 2020-12-31 | en |
| Abstract | Background: Dendrosomal nano-curcumin (DNC) represents an enormous potential to serve as a therapeutic anticancer agent. Here, we investigated the effect of DNC on wild type- and p53-mutant breast cancer cells. Methods: MCF-7 and T47D cells were treated with DNC and investigated for cell viability through MTT assay. The mode of death was analyzed using Annexin V/FITC staining and PARP cleavage assays. Flow cytometric efflux and cell swelling tests were employed to assess the p-glycoprotein (P-gp) transporter activity. Moreover, real-time PCR was employed to study the expression of Bax, Bcl-2 as well as survivin and its ΔEx3 splicing variant. Results: Our findings confirmed that DNC repressed cancer cell proliferation by induction of apoptosis. Additionally, DNC significantly modulated P-gp function that might lead to improved cellular permanence of curcumin. Malfunction of P-gp activity by DNC demonstrates its capability in reducing the drug resistance of p53-mutant cancer cells. Conclusions: In conclusion, our findings demonstrated that dendrosomal nano-curcumin could be considered an anti-tumor therapeutic for p53-mutant tumor malignancies. | en |
| DOI | https://doi.org/10.5812/jjcmb.109143 | en |
| Keyword | Curcumin | en |
| Keyword | Dendrosome Nanoparticles | en |
| Keyword | Apoptosis | en |
| Keyword | P-glycoprotein | en |
| Keyword | p53-mutant | en |
| Publisher | Brieflands | en |
| Title | Dendrosomal Nano-Curcumin Modulates P-Glycoprotein Activity and Induces Apoptosis in Wild Type and P53-Mutant Breast Cancer Cell Lines | en |
| Type | Research Article | en |