Oral Delivery of Insulin from Trimethyl Chitosan/Tragacanth: In Vitro, MTT, and In Vivo Bioactivity Tests
| Author | Alireza Khajeh-Amiri | en |
| Author | Ali Maghsoudi | en |
| Issued Date | 2023-03-31 | en |
| Abstract | Insulin-loaded Trimethyl Chitosan (TMC)/tragacanth microspheres were prepared by microemulsion dispersion and triggered in situ gelation. Microspheres were characterized by their mean size and distribution by laser diffraction spectroscopy and Brunauer-Emmet-Teller theory. Insulin encapsulation efficiency and in vitro release were determined by Bradford protein assay, and bioactivity was assessed in vitro using an enzyme-linked immunoassay diagnostic kit and in vivo using Wistar diabetic rats. TMC/tragacanth particles suppressed insulin release in acidic media and promoted a sustained release at near-neutral conditions. Micro-capsulated insulin was bioactive, demonstrated by both in vivo and in vitro bioassays. | en |
| DOI | https://doi.org/10.5812/amh-137796 | en |
| Keyword | Insulin | en |
| Keyword | Controlled Release | en |
| Keyword | Oral Delivery | en |
| Keyword | In Vivo Absorption | en |
| Publisher | Brieflands | en |
| Title | Oral Delivery of Insulin from Trimethyl Chitosan/Tragacanth: In Vitro, MTT, and In Vivo Bioactivity Tests | en |
| Type | Research Article | en |
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