Improvement of Antiproliferative Activity of Recombinant Truncated Form of Pseudomonas aeruginosa Exotoxin (PE38) by Vitamin E in MCF-7 Cells

Abstract

Background: In addition to beneficial roles of vitamin E in many metabolic processes and its antitumor activities, vitamin E derivatives have extensively been considered as permeability enhancers. Using these enhancers, permeability of a wide spectrum of drugs was reported to be significantly increased. PE38, a toxic substance with a potential application in cancer therapy, is a truncated form of Pseudomonas exotoxin A (PE), which lacks Ia and a portion of domain Ib. Objective: Here, the antiproliferative potential of PE38 and alpha-tocopherol (αT) form of vitamin E were assessed in MCF-7 cells. The role of vitamin E in PE38 cytotoxicity level was also evaluated. Materials and Methods: The antiproliferative potential of PE38, vitamin E, and a combination of them were colorimetrically evaluated in a cellular breast cancer model (MCF-7), using the MTT assay. P values of