Effect of Exercise Timing on Inflammation and Cognition in Aged Women with Type 2 Diabetes Mellitus: A Randomized Controlled Trial

AuthorAzra Mohammadpanah Ardakanen
AuthorZahra Hemati Farsanien
AuthorZahra Heydarien
AuthorShahrzad Habibi Ghahfarrokhien
AuthorZahra Raisien
OrcidAzra Mohammadpanah Ardakan [0000-0003-0259-5733]en
OrcidZahra Hemati Farsani [0000-0001-8499-795X]en
OrcidZahra Heydari [0009-0005-6862-7533]en
OrcidShahrzad Habibi Ghahfarrokhi [0000-0003-0817-5844]en
OrcidZahra Raisi [0000-0002-2741-0400]en
Issued Date2025-06-30en
AbstractBackground: This study aimed to elucidate the impact of exercise timing on inflammatory markers and cognitive function in elderly women with type 2 diabetes mellitus (T2DM), with a particular focus on the expression of proteins implicated in Alzheimer’s disease (AD) pathogenesis, including TYRO protein tyrosine kinase-binding protein (TYROBP), soluble triggering receptor expressed on myeloid cells 2 (sTREM2), transforming growth factor beta (TGF-β), apolipoprotein E (APOE), and Osteopontin (SPP1). Objectives: This research aimed to address: Which exercise time — morning or evening — provides greater health benefits for elderly women with T2DM, particularly regarding key health indicators? Methods: This study was a randomized controlled trial. A total of 45 women, aged 55 - 65 years, diagnosed with T2DM, were randomly assigned to morning exercise, evening exercise, or control groups using a computer-generated random number sequence. The tool used in this study is the Montreal Cognitive Function Questionnaire. The intervention consisted of a 12-week aerobic exercise (TPMP) regimen, calibrated to 60 - 70% of the participants’ maximum heart rate reserve. Protein expression levels were quantified pre- and post-intervention using enzyme-linked immunosorbent assay (ELISA). To mitigate bias, outcome assessors were blinded to participant group allocation via a data coding protocol. Results: The results of MANCOVA and ANCOVA showed that aerobic activities in the morning and evening have a significant effect on cognitive performance in older adults (F = 14.17, P < 0.05); and the effects of exercise training in the evening hours are greater. Also, morning exercise was associated with a statistically significant reduction in TGF-β and SPP1 levels (P < 0.05), whereas evening exercise significantly decreased TYROBP and sTREM2 levels (P < 0.01). Notably, APOE levels were markedly elevated in the evening exercise group, with a significant increase compared to the control group (P < 0.05) and the morning exercise group (P < 0.01). Conclusions: The findings suggest that a structured TPMP program, regardless of timing, can beneficially modulate key inflammatory markers associated with AD in women with T2DM. These results highlight the potential for exercise as a non-pharmacological intervention to mitigate AD risk in this vulnerable population.en
DOIhttps://doi.org/10.5812/ijpbs-158682en
URIhttps://brieflands.com/journals/ijpbs/articles/158682en
KeywordAgeden
KeywordCognitionen
KeywordExerciseen
KeywordInflammationen
KeywordType 2 Diabetes Mellitusen
KeywordWomenen
PublisherBrieflandsen
TitleEffect of Exercise Timing on Inflammation and Cognition in Aged Women with Type 2 Diabetes Mellitus: A Randomized Controlled Trialen
TypeResearch Articleen

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