Expression of Receptor Binding Domain (RBD) from Coronavirus Spike Protein Fused to Carboxylic Terminal of Clostridium perfringens Enterotoxin (c-CPE) in Pichia pastoris

Abstract

Background: To enhance mucosal IgA, administering intra-nasal antigen transfer may be considered. One challenge with this approach is antigenic factors' limited penetration of nasal mucosa. This research utilized c-CPE, the carboxylic end-section of the enterotoxin Clostridium perfringens. Specifically, the RBD-c-CPE fusion structure was cloned in a bacterial host and integrated into the yeast host genome for protein expression.