Expression of Receptor Binding Domain (RBD) from Coronavirus Spike Protein Fused to Carboxylic Terminal of Clostridium perfringens Enterotoxin (c-CPE) in Pichia pastoris
| Author | Elham Behvandi | en |
| Author | Ghasem Bagherpour | en |
| Author | Keyvan Nedaei | en |
| Author | Saeid Kaboli | en |
| Author | Behrooz Johari | en |
| Issued Date | 2024-03-31 | en |
| Abstract | Background: To enhance mucosal IgA, administering intra-nasal antigen transfer may be considered. One challenge with this approach is antigenic factors' limited penetration of nasal mucosa. This research utilized c-CPE, the carboxylic end-section of the enterotoxin Clostridium perfringens. Specifically, the RBD-c-CPE fusion structure was cloned in a bacterial host and integrated into the yeast host genome for protein expression. | en |
| DOI | https://doi.org/10.69107/koomesh-149316 | en |
| Keyword | Recombinant Fusion Proteins | en |
| Keyword | Cloning | en |
| Keyword | Spike Protein | en |
| Keyword | Pichia pastoris | en |
| Publisher | Brieflands | en |
| Title | Expression of Receptor Binding Domain (RBD) from Coronavirus Spike Protein Fused to Carboxylic Terminal of Clostridium perfringens Enterotoxin (c-CPE) in Pichia pastoris | en |
| Type | Research Article | en |