Role of Bax Protein and Caspase-3 at High Glucose-Induced Apoptosis in Human Embryonic Kidney (HEK) 293 Cells
| Author | Habib Islamidulabi | en |
| Author | Ali Mohammad Sharifi | en |
| Issued Date | 2013-05-31 | en |
| Abstract | Background : Hyperglycemia is the principal factor responsible for microvascular complications of diabetes. Diabetic nephropathy is a serious and common complication leading to end stage renal disease. The exact molecular mechanisms of high glucose-induced toxicity on renal cells are still incompletely understood. Therefore in the present study, glucose-induced toxicity was studied in HEK (human embryonic kidney) cells as an in vitro model for diabetic nephropathy.Materials and Methods : First, the viability of HEK 293 cells exposed to glucose was measured by MTT (Methyl Thiazolyl Tetra-zolium) assay. Caspase-3 activity was determined spectrophotometrically using enzyme specific substrate. Moreover, the alteration in expression of Bax, Bcl2 and caspase-3 were measured by Western blotting.Results : The results showed that high glucose significantly reduced cell viability after 48 h (p | en |
| DOI | https://doi.org/ | en |
| Keyword | High glucose | en |
| Keyword | HEK293 cells | en |
| Keyword | Apoptosis | en |
| Keyword | Bax | en |
| Keyword | Bcl-2 | en |
| Keyword | Caspase-3 | en |
| Publisher | Brieflands | en |
| Title | Role of Bax Protein and Caspase-3 at High Glucose-Induced Apoptosis in Human Embryonic Kidney (HEK) 293 Cells | en |
| Type | Research Article | en |
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