Pro-Oxidant and Antioxidant Balance, Anthropometric Parameters, and Nutrient Intakes in Gastro-Esophageal Reflux Disease Patients

Abstract

Background and Objectives: Gastro-esophageal reflux disease (GERD) is a common syndrome. Several factors are known with GERD, which lead to improvement in reactive oxygen species (ROS). The objective of this study was to determine pro-oxidant and antioxidant balance (PAB) in GERD patients in order to determine oxidative stress and the correlation between PAB and other parameters in the population study. Methods: Overall, 38 GERD patients (mean age 31.3 ± 10.1 years) and 60 healthy subjects (mean age 30.2 ± 14.8 years) were selected as controls. After recording the demographic characteristics of patients, body weight, height, waist circumference (WC), and hip circumference were determined as well as body mass index (BMI), waist to hip ratio (WHpR), and body size were calculated. Food frequency questionnaire was used for data about food consumption. Information on food habits was recorded by a trained interviewer. The PAB was simultaneously measured by a modified PAB assay using 3,3’,5,5’-tetramethylbenzidine benzidine (TMB). Statistical analysis was done by the SPSS software, version 17. Data were considered significant at a level of P < 0.05. Results: The results showed that there was not a significant difference based on the mean levels according to PAB, food groups, and food habits between the two groups. There was a significant correlation between PAB and BMI (r = 0.41, P = 0.0001) and WC r = 0.24, P = 0.04). There was a significant difference based on consumption of fast foods between the two groups (X2 = 12.3, P = 0.002). Conclusions: The findings showed that PAB values are not significantly increased in GERD patients compared with controls. However, they are exposed to oxidative stress. A significant correlation was observed between PAB and BMI, and WC. It seems the increase of PAB in these patients needs to be confirmed by further studies.