Development of Agglutination Test for Detection of isolated Mannoprotein Antigen from Candida Albicans

Abstract

Background: Candida albicans is one of the most common causes of hospital infections. The aim of this study was to develop a rapid, easy and sensitive way to detect candida albicans. Material and Methods: Polyclonal antibodies were prepared in rabbits. The antibodies were purified by salt concentration and Ion exchange chromatography. The purified antibody was coated onto the colloidal gold. Results: Color change (red to blue) was observed when the purified antigens (mannoprotein of Candida albicans cell wall) were added to Polyclonal antibodies. The method was sensitive and easy. Conclusion: This study indicated that using colloidal gold particle agglutination method can be used for accurate and rapid candida detection Method.