Development of Agglutination Test for Detection of isolated Mannoprotein Antigen from Candida Albicans
| Author | Masoumeh Rajabibazl | en |
| Author | Mohammad Javad Rasaee | en |
| Author | Mahtab Nouri Fard | en |
| Author | Zohreh Farahnezhad | en |
| Issued Date | 2013-06-30 | en |
| Abstract | Background: Candida albicans is one of the most common causes of hospital infections. The aim of this study was to develop a rapid, easy and sensitive way to detect candida albicans. Material and Methods: Polyclonal antibodies were prepared in rabbits. The antibodies were purified by salt concentration and Ion exchange chromatography. The purified antibody was coated onto the colloidal gold. Results: Color change (red to blue) was observed when the purified antigens (mannoprotein of Candida albicans cell wall) were added to Polyclonal antibodies. The method was sensitive and easy. Conclusion: This study indicated that using colloidal gold particle agglutination method can be used for accurate and rapid candida detection Method. | en |
| DOI | https://doi.org/ | en |
| Keyword | Candida Albicans | en |
| Keyword | Mannoproteins | en |
| Keyword | Antigen | en |
| Keyword | Agglutination | en |
| Keyword | Gold | en |
| Publisher | Brieflands | en |
| Title | Development of Agglutination Test for Detection of isolated Mannoprotein Antigen from Candida Albicans | en |
| Type | Research Article | en |
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