Standardization of Molecular Diagnostic of the entC and ent E Staphylococcus aureus Isolated from Human Infections in Zabol
Author | Khadije Rezaie Keikhaie |
Author | Gholamreza Bagheri |
Author | Atefeh Kamali |
Author | Saphora Bazi |
Author | Zahra Shahi |
Author | Fereshteh Javadian |
Accessioned Date | 2023-12-24T18:45:21Z |
Available Date | 2023-12-24T18:45:21Z |
Issued Date | 2017-10-31 |
Abstract | Background: Staphylococcus aureus is one of the most important causes of hospital infections around the world. It has been found in various studies, that 15% - 18% of the Staphylococcus aureus strains isolated from different sources are capable of producing enterotoxin. The aim of this study is identifying Staphylococcus aureus, types C and E, which produce Enterotoxin, from human Infections in Zabol by PCR. Methods: The present study was conducted on 60 urine samples in a 1-year period. Samples were randomly collected from different parts of the city of Zabol and transferred to the university lab in a minimum period of time by maintaining cold conditions. DNA extraction was performed and PCR was done for enterotoxin E and C. Results: The results of this study showed that isolated S. aureus was resistant to antibiotics including cefazolin (7.3%), doxycycline (12.10%), erythromycin (22.3%), rifampin (9.7%), ciprofloxacin (18.10%), and Co-trimoxazole (14.5%). The results of this study showed that the prevalence of enterotoxin E gene in S. aureus samples was 1.5%. Conclusions: Regarding the importance of S. aureus secretes, 2 types of toxins with super antigen activity, enterotoxins, of which there are 6 antigenic types (named SE-A, B, C, D, E and G). In case of expression of enterotoxin genes, rapid treatment of the infection seems to be necessary. |
DOI | https://doi.org/ 10.5812/gct.59214 |
URI | https://repository.brieflands.com/handle/123456789/36372 |
Keyword | Enterotoxin |
Keyword | PCR |
Keyword | Staphylococcus aureus |
Publisher | Brieflands |
Title | Standardization of Molecular Diagnostic of the entC and ent E Staphylococcus aureus Isolated from Human Infections in Zabol |
Type | Research Article |
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